Fluorescent compounds should be protected from light for long term storage. Storage conditions can be found on the product information sheet or product safety and data sheet, material safety data sheet, and on the product label. However, the majority of these products are highly stable for many years, as long as they are stored as recommended. If you have an older conjugate in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.įor lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates).īiotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples. Kits often are functional for significantly longer than the guaranteed shelf life. Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. 23017) can be used to mount skeletal muscle sections stained with bungarotoxin.Ĭy Dye is a registered trademark of Cytiva. We have, however, found that EverBrite TrueBlack® Mounting Medium (cat. While we would usually recommend our TrueBlack® lipofuscin quenchers for human brain tissue, they are not compatible with bungarotoxin staining. This usually shows up as bright, punctate dots around cell nuclei. However, if you are staining human tissue (especially brain), lipofuscin autofluorescence may be bright in all channels. While the tissue shows autofluorescence, the bungarotoxin staining of motor endplates is usually much brighter than the background for all of the dye colors we’ve tested. We test fluorescent bungarotoxin on rat skeletal muscle sections. 00009) is a far-red conjugate for the Cy®5 channel with a low negative charge that should have low background from either the dye or autofluorescence. Endogenous fluorescence background in tissue is usually highest in the blue wavelengths (DAPI channel) and lowest in the far-red (Cy®5 channel). When staining tissues, the endogenous autofluorescence of the tissue itself is often the most significant source of background. Usually, this is more of a concern with labeled antibodies that carry many dyes, as opposed to a small toxin like bungarotoxin. Opal is a trademark of Akoya Biosciences.ĭyes that carry multiple negative charges can introduce background. Note: The CF® Dyes listed here are spectrally similar alternatives to Opal™ dyes, they have not been validated as direct replacements for use with Akoya's kits or imaging system. CF® Dye Alternatives for Akoya Tyramides Akoya Tyramide They have not been validated as direct replacements for use with Akoya kits or imaging systems. Unless noted as a direct replacement, these tyramide dyes are spectrally similar alternatives. See below for our recommended alternatives to Akoya tyramides.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |